The Genomics & Transcriptomics Labor (GTL) at HHU Dusseldorf
Direct link to the GTL LIMS login here.
Our NGS portfolio
DNA
- Whole genome sequencing
- Illumina TruSeq PCR-free
- Illumina TruSeq DNA nano
- Ilumina Nextera Flex (also with cells)
- AmpliconDeepSeq
- small genome/long-range PCR products
- linked read WGS (TellSeq)
- Whole exome sequencing
- SureSelect v6 and v7
- HS protocol with UMI for cfDNA
- Including FFPE repair
- Gene Panel sequencing (>50 genes, hybridization based)
- small gene panel (amplicon- based)
RNA
- mRNAseq
- total RNAseq incl. ribo-depletion
- 3´RNAseq
- low-input RNAseq
- with Nugen Ovation
- with Nugen SoLo
- circSeq, R2C2
- transient RNASeq (e.g. 4sU, SLAMseq)
- RiboSeq
- Gene Panel sequencing (on cDNA e.g. as for COVID research)
EPI
- ChiP-seq
- Standard with Ligation
- DamID
- CUT&RUN
- CUT&Tag (Chipmentation)
- miRNAseq
- methylSeq
- WGBS
- EM-Seq
- cfNOMe (including methylation and nucleosome footprints)
- Targeted MethySeq (hybridization-based)
- RIP-seq, PARclip, eCLIp
Long reads
- Oxford Nanopore Technologies
- Direct cDNA Sequencing
- Genome Ligation
- Genome Rapid w&w/o PCR
- mRNA Seq PCR & PCR-free
- Direct mRNA Seq
- Ultra Long
- 1D² Sequencing Kit
- Oxford Nanopore Technologies/PacBio
- Full Length 16S
- Amplicons
- PacBio
- mRNA Sequencing (Iso-Seq)
- WGS – HiFi (15-20 kb)
- WGS – CLR (>30 kb)
- WGS – Low & Ultra-Low Input
- Shotgun Metagenomics
- No-Amp Targeted Sequencing
- Bionano
- Optical Mapping
Single cell
- 10x Chromium
- 3´snRNAseq
- 3´and 5´scRNAseq
- TCR-seq
- BCR-seq
- full-length scRNAseq
- with ICell 8
- with SMARTseq 3
- Spatial transcriptomics
- scATAC
- scCNV
- scEMSeq (dev.)
- BD Rhapsody (PRECISE)
- SeqWell V1, S3 (PRECISE)
- Ab-Seq (PRECISE)